By this re amp kit one can obtain 1 3 µg gdna from 1 10 ng previous malbac amplified products in about 1 hour.
Malbac single cell wga kit.
With 30x sequencing depth 93 of the single cell genome can be covered compared with 50 60 by mda.
Whole genome amplification from a single cell is now possible with our optimized genomeplex single cell whole genome amplification kit the single cell procedure differs very little from the previously described genomeplex system but for three procedural changes.
Three methods are commonly used for wga.
The re amped products keep good fidelity to the first round malbac products without introducing further amplification bias.
For ffpe samples the amplification efficiency and reproducibility is limited.
The kit is able to achieve consistent amplification efficiency in both at.
Therefore we recommend at least 100 cells used for analyzing ffpe samples by malbac wga.
The single cell wga kit may not be transferred to third parties resold modified for resale used to provide commercial services or used to manufacture commercial products without written approval of yikon genomics co.
1 the kit includes a robust optimized cell lysis protocol that is incorporated into the.
Therefore whole genome ampli cation wga.
Qpcr microarray and high throughput sequencing often require the sample to meet speci c requirements for quantity and quality.
The single cell wga kit exhibits significantly improved amplification evenness compared with mda.
Malbac single cell wga kit malbac single cell wga kit rapid reliable and reproducible whole genome ampli cation for single cells nucleic acid analytical platforms e g.
The single cell wga kit is intended to be used for research purposes only.
The malbac single cell wga kit performs well on cultured cells white blood cells facs sorted cells fresh or frozen tissues and formaldehyde fixed tissues and cells.
Malbac single cell wga kit is based on the novel patented malbac technology and provides rapid reliable and reproducible whole genome amplification from single cells or equivalent gdna picogram level to produce 2 4 micrograms of amplified gdna in about 4 hours.
Multiple displacement amplification mda degenerate oligonucleotide primed pcr dop pcr and multiple annealing and looping based amplification cycles malbac.
Single cell resequencing scrs provides many biomedical advances in variations detection at the single cell level but it currently relies on whole genome amplification wga.
Malbac single cell wga kit.
Whole genome amplification for single cell s picogram to sub nanogram quantities of gdna.
Malbac single cell wga kit provides rapid reliable and reproducible whole genome amplification of single cell s or picogram to sub nanogram quantities of gdna to produce 2 4 micrograms of amplified products in about 4 hours.
